3D printing the first ever biomimetic tongue surface
Scientists have created synthetic soft surfaces with tongue-like textures for the first time using 3D printing.
This opens new possibilities for testing oral processing properties of food, nutritional technologies, pharmaceutics and dry mouth therapies.
UK scientists led by the University of Leeds in collaboration with the University of Edinburgh have replicated the highly sophisticated surface design of a human tongue and demonstrated that their printed synthetic silicone structure mimics the topology, elasticity and wettability of the tongue’s surface.
These factors are instrumental to how food or saliva interacts with the tongue, which in turn can affect mouthfeel, swallowing, speech, nutritional intake and quality of life.
Particularly, since the onset of the COVID-19 pandemic, social distancing has posed significant challenges to carry out such sensory trials and consumer tests. A biomimetic tongue will be immensely helpful to increase development productivity and reducing manufacturers’ reliance on human trials in the early stages.
The complex nature of the tongue’s biological surface has posed challenges in artificial replication, adding major obstacles to the development and screening of effective long-lasting treatments or therapies for dry mouth syndrome — roughly 10% of the general population and 30% of older people suffer from dry mouth.
Study lead author, Dr Efren Andablo-Reyes conducted this research while a postdoctoral fellow in the School of Food Science and Nutrition at Leeds. He said: “Recreating the surface of an average human tongue comes with unique architectural challenges. Hundreds of small bud-like structures called papilla give the tongue its characteristic rough texture that in combination to the soft nature of the tissue create a complicated landscape from a mechanical perspective.
“We focused our attention on the anterior dorsal section of the tongue where some of these papillae contain taste receptors, while many of them lack such receptors.
“Both kinds of papillae play a critical role in providing the right mechanical friction to aid food processing in the mouth with the adequate amount saliva, providing pleasurable mouthfeel perception and proper lubrication for swallowing.
“We aimed to replicate these mechanically relevant characteristics of the human tongue in a surface that is easy to use in the lab to replicate oral processing conditions.”
The team took silicone impressions of tongue surfaces from fifteen adults. The impressions were 3D optically scanned to map papillae dimensions, density and the average roughness of the tongues. The texture of a human tongue was found to resemble a random layout.
Silicone impressions of human palate and tongue.
Silicone impressions of human palate and tongue. Human tongue impressions were collected from 15 healthy subjects in the morning, at least two hours since eating and drinking. Credit: Anwesha Sarkar
The team used computer simulations and mathematical modelling to create a 3D-printed artificial surface to function as a mould containing wells with the shape and dimensions of the different papillae randomly distributed across the surface with right density. This was replica-moulded against elastomers of optimised softness and wettability.
University of Edinburgh co-author, Rik Sarkar of the School of Informatics said: “The randomness in distribution of papillae appears to play an important sensory role for the tongue.
“We defined a new concept called collision probability to measure mechanosensing that will have large impact in this area. In the future, we will use a combination of machine learning and computational topology to create tongue models of diverse healthy and diseased individuals to address various oral conditions.”
The artificial surface was then 3D printed using digital light processing technology based in the School of Mechanical Engineering at Leeds.