Seamer Jack Blatherwick has been added to an otherwise unchanged squad to face Glamorgan at Sophia Gardens.
The Red Rose head into the clash top of Group Three, following a thrilling Roses victory over Yorkshire last week.
Lancashire and Glamorgan met in the reverse fixture last month, a rainy draw at Emirates Old Trafford.
Lancashire squad to face Glamorgan
Dane Vilas (c), Tom Bailey, Jack Blatherwick, Josh Bohannon, Steven Croft, Alex Davies, Keaton Jennings, Danny Lamb, Liam Livingstone, Saqib Mahmood, Matt Parkinson, Luke Wells, Luke Wood